UNLOCKING THE BOTTLENECK IN FORWARD GENETICS USING WHOLE-GENOME SEQUENCING AND IDENTITY BY DESCENT TO ISOLATE CAUSATIVE MUTATIONS.

Unlocking the bottleneck in forward genetics using whole-genome sequencing and identity by descent to isolate causative mutations.

Forward genetics screens with N-ethyl-N-nitrosourea (ENU) provide a powerful way to illuminate gene function and generate mouse models of human disease; however, the identification of causative mutations remains a limiting step.Current strategies depend on conventional mapping, so the propagation of Tungsten Carbide Burrs affected mice requires non

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